forwardmappit

ForwardMAPPIT

In the basic lay-out of ForwardMAPPIT, the bait is fused to a signalling-deficient receptor chimera and the prey is tethered to a receptor fragment containing functional STAT3 recruitment sites. Bait-prey interaction reconstitutes a functional receptor complex which, upon ligand binding, initiates the JAK-STAT signalling cascade. For analysis of the interaction between designated protein pairs, the ForwardMAPPIT mode can be combined with a binary assay. For detecting new interactions, FACS or array screening assays are available.    A new focus is on the development of FACS-based MAPPIT strategies to test virtually all possible point mutants of a protein against all its interactions that are detectable via MAPPIT for large-scale "edgetics" studies. Similar strategies are being developed to study very large mutant panels in multiple signaling assays in parallel.

More information:
Related publications (methods):
          descibes a random mutagenesis approach to identify interaction sites.
Related publications (protocol):
Related publications (applications):