forwardmappit

ForwardMAPPIT

In the basic lay-out of ForwardMAPPIT, the bait is fused to a signalling-deficient receptor chimera and the prey is tethered to a receptor fragment containing functional STAT3 recruitment sites. Bait-prey interaction reconstitutes a functional receptor complex which, upon ligand binding, initiates the JAK-STAT signalling cascade. For analysis of the interaction between designated protein pairs, the ForwardMAPPIT mode can be combined with a binary assay. For detecting new interactions, FACS or array screening assays are available.

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