A limitation of MAPPIT is that the interaction detection zone is restricted to the plasmamembrane environment, and that it is incompatible with full size transmembrane proteins. To overcome these restrictions KISS (for KInase Substrate Sensor) was developed, an adaptation from MAPPIT whereby the signaling incompetent receptor is replaced by the kinase domain of Tyk2, a JAK2 family member. Since KISS uses the same prey configuration and read-out as MAPPIT, the array screening platform is transferable to the KISS assay.

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