MAPPIT opportunities
MAPPIT features
- operates in intact human cells, the optimal physiological context for studying human proteins
- functions in the presence of endogenous proteins, allowing for the detection of indirect interactions
- allows the detection of modification-dependent interactions
- allows the detection of weak and transient interactions
- allows the study of the dynamics of protein-protein interactions
- works in different cell types, including epithelial, hematopoietic and neuronal cells
- is ligand-inducible, adding an extra level of control over false-positives
- can be used both for binary analyses of protein interactions and screening of complex cDNA libraries
- makes use of simple read-outs, making it suitable for large-scale analyses
- can be used both for the analysis of interactions among proteins and interactions between proteins and other organic molecules
MAPPIT applications
The MAPPIT technology suite offers a set of highly complementary tools for the analysis of interactions among proteins or between proteins and small organic molecules in mammalian cells. In basic research it is being applied in interactome analysis, both for the detailed examination of designated protein-protein interactions, for cDNA library screening experiments and for systematic large scale interactome mapping projects.
Through the combination of its three core techniques, MAPPIT constitutes a valuable platform for drug discovery and pre-clinical analysis of compounds that act by disrupting protein-protein interactions, a novel class of therapeutics. ForwardMAPPIT allows for the identification of target protein interactions. Using ReverseMAPPIT, small molecule libraries can be screened for compounds that interfere with these target interactions. MASPIT permits target profiling and optimization of the identified lead compounds.
A selection of example MAPPIT applications can be found here.
